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Crotalus durissus terrificus snake venom regulates macrophage metabolism and function.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 2001 Oct; Vol. 70 (4), pp. 551-8. - Publication Year :
- 2001
-
Abstract
- In the present study, we examined the effect of Crotalus durissus terrificus venom on rat macrophage metabolism and function. Two hours after subcutaneous injection of the venom, peritoneal resident (unstimulated), elicited (thioglycollate-stimulated), and activated Mycobacterium bovis strain bacille Calmette Guérin (BCG) macrophages were collected, and their functional and metabolic parameters were analyzed. The venom inhibited spreading and phagocytosis of macrophages. On the other hand, this treatment increased H(2)O(2) and NO production, candidacidal activity, and the activities of key enzymes of glycolysis and glutaminolysis. We also investigated whether the venom could affect macrophage activation by thioglycollate or BCG. The administration of venom 2 h before injection of thioglycollate and BCG or 2 or 3 days after injection of the thioglycollate or BCG, respectively, did not modify the previous observations. These findings suggest that crotalic venom leads the macrophage to an activated state, with high production of oxygen- and nitrogen-reactive species. This cell activation state does not include inflammatory properties of spreading and phagocytosis.
- Subjects :
- Animals
Candida albicans physiology
Cell Count
Cells, Cultured
Glucose metabolism
Glutamine metabolism
Hydrogen Peroxide metabolism
Macrophages, Peritoneal drug effects
Male
Mycobacterium bovis physiology
Nitric Oxide biosynthesis
Phagocytosis drug effects
Rats
Rats, Wistar
Thioglycolates pharmacology
Time Factors
Crotalid Venoms pharmacology
Crotalus
Macrophage Activation drug effects
Macrophages, Peritoneal immunology
Macrophages, Peritoneal metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0741-5400
- Volume :
- 70
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 11590191