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cAMP is involved in the differentiation of human teratocarcinoma cells.
- Source :
-
Neuroscience letters [Neurosci Lett] 2001 Sep 21; Vol. 311 (1), pp. 61-5. - Publication Year :
- 2001
-
Abstract
- The generation of fully differentiated post-mitotic human neuronal cells from stem cells (human teratocarcinoma (hNT2) cells) might enable the development of a co-culture model of human neurons with human Schwann cells (SCs). This co-culture model is an important tool to study formation of myelin sheaths. However, the thin process of the post-mitotic human neuronal cells formed under known culture conditions do not provide a good substrate for human SCs to start myelination. We optimized the culture conditions of these cells to obtain axons with a larger diameter. Western blotting and immunofluorescence studies were performed to confirm the neuronal status of the cells and diameter of the processes. In this study, we show that addition of cAMP-inducing factors to hNT2 cells resulted in rapid morphological changes including the development of processes with a larger diameter.
- Subjects :
- 1-Methyl-3-isobutylxanthine pharmacology
Cell Culture Techniques methods
Cell Differentiation physiology
Cell Size drug effects
Cell Size physiology
Coculture Techniques methods
Colforsin pharmacology
Embryonal Carcinoma Stem Cells
Humans
Insulin pharmacology
Myelin Sheath ultrastructure
Neoplastic Stem Cells cytology
Neuregulin-1 pharmacology
Neurites drug effects
Neurites metabolism
Neurites ultrastructure
Neurons cytology
Phosphodiesterase Inhibitors pharmacology
Tretinoin pharmacology
Cell Differentiation drug effects
Cyclic AMP metabolism
Myelin Sheath metabolism
Neoplastic Stem Cells drug effects
Neoplastic Stem Cells metabolism
Neurons drug effects
Neurons metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3940
- Volume :
- 311
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 11585568
- Full Text :
- https://doi.org/10.1016/s0304-3940(01)02108-5