Upregulation of matrix metalloproteinase-1 production by prostaglandin F2alpha in human gingival fibroblasts.

In the present study, we investigated the effect of prostglandin F2alpha (PGF2alpha) and combination of PGF2alpha and interleukin(IL)-1beta on matrix metalloproteinase (MMP)-1 production in human gingival fibroblasts (HGF). PGF2alpha enhanced MMP-1 production in a dose-dependent manner in HGF. Combination of PGF2alpha and IL-1beta induced a synergistic increase of MMP-1 production in HGF. Furthermore, fluprostenol, a specific FP receptor agonist, increased MMP-1 production and induced a synergistic enhancement of IL-1beta-induced MMP-1 production in HGF, similar to PGF2alpha. FP receptor mRNA expression was detected in HGF, by reverse transcription-polymerase chain reaction. Northern blot analysis revealed that PGF2alpha enhanced MMP-1 mRNA expression in HGF and that PGF2alpha increased MMP-1 mRNA levels induced by IL-1beta. In conclusion, we suggest that PGF2alpha increases MMP-1 production in HGF and synergistically enhances MMP-1 production in IL-1beta-stimulated HGF. PGF2alpha may be involved in degradation of connective tissue in periodontal lesions.