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Recombineering: a powerful new tool for mouse functional genomics.
- Source :
-
Nature reviews. Genetics [Nat Rev Genet] 2001 Oct; Vol. 2 (10), pp. 769-79. - Publication Year :
- 2001
-
Abstract
- Highly efficient phage-based Escherichia coli homologous recombination systems have recently been developed that enable genomic DNA in bacterial artificial chromosomes to be modified and subcloned, without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombinogenic engineering or recombineering, is efficient and greatly decreases the time it takes to create transgenic mouse models by traditional means. Recombineering also facilitates many kinds of genomic experiment that have otherwise been difficult to carry out, and should enhance functional genomic studies by providing better mouse models and a more refined genetic analysis of the mouse genome.
- Subjects :
- Animals
Bacterial Proteins physiology
Bacteriophage P1 genetics
Bacteriophage lambda genetics
Chromosomes, Artificial, Bacterial genetics
Chromosomes, Artificial, P1 Bacteriophage genetics
Chromosomes, Artificial, Yeast genetics
Cloning, Molecular methods
DNA Repair
DNA, Bacterial genetics
DNA, Fungal genetics
DNA, Recombinant genetics
DNA, Single-Stranded genetics
Escherichia coli genetics
Exodeoxyribonuclease V
Exodeoxyribonucleases physiology
Forecasting
Gene Expression Regulation, Viral
Mice, Knockout
Mice, Transgenic
Rec A Recombinases metabolism
Regulatory Sequences, Nucleic Acid
Saccharomyces cerevisiae genetics
Sequence Homology, Nucleic Acid
Transgenes
Viral Proteins physiology
DNA-Binding Proteins
Escherichia coli Proteins
Genetic Engineering methods
Genomics methods
Mice genetics
Recombination, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1471-0056
- Volume :
- 2
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Nature reviews. Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 11584293
- Full Text :
- https://doi.org/10.1038/35093556