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A potential autocrine pathway for growth hormone releasing hormone (GHRH) and its receptor in human prostate cancer cell lines.
- Source :
-
The Prostate [Prostate] 2001 Oct 01; Vol. 49 (2), pp. 116-21. - Publication Year :
- 2001
-
Abstract
- Background: Recent studies have shown that GHRH antagonists inhibit prostate tumour growth and IGF-II production both in vivo and in vitro. The mechanism underlying these observations is unknown, but may involve an interaction with a prostatic GHRH receptor (GHRH-R), raising the possibility of an autocrine pathway for the GHRH axis in the prostate.<br />Methods: GHRH and GHRH-R mRNA expression was examined by RT-PCR in human prostate cancer cell lines, and the authenticity of PCR products was confirmed by Southern analysis and cDNA sequencing. Immunohistochemical techniques were used to examine the expression of GHRH protein in prostate cancer cell lines.<br />Results: GHRH-R (mRNA) and GHRH (mRNA and protein) are co-expressed in the ALVA-41, DU145, LNCaP and PC3 human prostate cancer cell lines.<br />Conclusions: These observations suggest the presence of an intact prostatic GHRH autocrine pathway which may stimulate prostate cell proliferation. This pathway may be disrupted by GHRH antagonists.<br /> (Copyright 2001 Wiley-Liss, Inc.)
- Subjects :
- Blotting, Southern
Cell Division physiology
DNA, Neoplasm biosynthesis
DNA, Neoplasm genetics
Growth Hormone-Releasing Hormone genetics
Humans
Immunohistochemistry
Male
Prostatic Neoplasms genetics
RNA, Messenger biosynthesis
RNA, Messenger genetics
Receptors, Neuropeptide genetics
Receptors, Pituitary Hormone-Regulating Hormone genetics
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Tumor Cells, Cultured
Growth Hormone-Releasing Hormone biosynthesis
Prostatic Neoplasms metabolism
Prostatic Neoplasms pathology
Receptors, Neuropeptide biosynthesis
Receptors, Pituitary Hormone-Regulating Hormone biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0270-4137
- Volume :
- 49
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Prostate
- Publication Type :
- Academic Journal
- Accession number :
- 11582590
- Full Text :
- https://doi.org/10.1002/pros.1125