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A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase.
- Source :
-
Nucleosides, nucleotides & nucleic acids [Nucleosides Nucleotides Nucleic Acids] 2001 Sep; Vol. 20 (9), pp. 1647-54. - Publication Year :
- 2001
-
Abstract
- By affinity chromatography with Sepharose coupled to 2'-deoxy-1-beta-D-ribofuranosyl-N4-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated.
- Subjects :
- Animals
Carcinoma, Ehrlich Tumor enzymology
Chromatography, High Pressure Liquid
Cytidine Deaminase metabolism
Deoxycytidine Kinase metabolism
Kinetics
Mice
Sepharose analogs & derivatives
Sepharose metabolism
Tumor Cells, Cultured
Chromatography, Affinity methods
Cytidine Deaminase isolation & purification
Deoxycytidine Kinase isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1525-7770
- Volume :
- 20
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Nucleosides, nucleotides & nucleic acids
- Publication Type :
- Academic Journal
- Accession number :
- 11580191
- Full Text :
- https://doi.org/10.1081/NCN-100105901