Back to Search
Start Over
Sporadic testicular germ cell cancers do not exhibit specific alteration in CAG/CTG repeats containing genes expressed in human testis.
- Source :
-
Oncogene [Oncogene] 2001 Sep 06; Vol. 20 (39), pp. 5548-53. - Publication Year :
- 2001
-
Abstract
- CAG/CTG repeat expansions in genomic DNA of testicular tumour cell lines, and germline DNA from members of families predisposed to this malignancy, have been previously described. In order to identify genes possibly concerned by this alteration, we attempted to clone all possible human testis cDNA containing at least five CAG/CTG repeats. Thirty-four different transcripts were identified. By using PCR and non denaturing gel electrophoresis, we determined the size of their repeats, as well as their polymorphisms in a collection of human testicular germ cell tumours and the normal surrounding tissues. For all tested genes, we detected the presence of several species of the same mRNA for each person. Nine genes exhibited specific patterns of expression among different groups of individuals, indicative of polymorphism. None of these polymorphisms was related to human testicular tumours.
- Subjects :
- Anticipation, Genetic
DNA, Neoplasm genetics
Gene Expression Regulation, Neoplastic
Genes
Germinoma metabolism
Humans
Male
Polymorphism, Genetic
RNA, Messenger biosynthesis
RNA, Neoplasm biosynthesis
Testicular Neoplasms metabolism
Transcription, Genetic
Germinoma genetics
Testicular Neoplasms genetics
Testis metabolism
Trinucleotide Repeat Expansion
Subjects
Details
- Language :
- English
- ISSN :
- 0950-9232
- Volume :
- 20
- Issue :
- 39
- Database :
- MEDLINE
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 11571654
- Full Text :
- https://doi.org/10.1038/sj.onc.1204714