Properties of arabinonucleic acids (ANA & 20'F-ANA): implications for the design of antisense therapeutics that invoke RNase H cleavage of RNA.

Authors :: Damha MJ
Noronha AM
Wilds CJ
Trempe JF
Denisov A
Pon RT
Gehring K
Source :: Nucleosides, nucleotides & nucleic acids [Nucleosides Nucleotides Nucleic Acids] 2001 Apr-Jul; Vol. 20 (4-7), pp. 429-40.
Publication Year :: 2001
Abstract: Inversion of configuration of the C2' position of RNA leads to a very unique nucleic acid structure: arabinonucleic acid (ANA). ANA, and its 2'-fluoro derivative (2'F-ANA) from hybrids with RNA that are capable of activating RNase H, resulting in cleavage of the RNA strand. In this paper, we review the properties of duplexes formed between ANA (or 2'F-ANA) and its RNA complement. These studies support the notion that RNase H is sensitive to the minor groove dimensions of the hybrid substrate.

Subjects :: Models, Molecular
Nucleic Acid Conformation
RNA metabolism
Ribonuclease H metabolism
Stereoisomerism
Arabinonucleotides chemistry
RNA chemistry
RNA, Antisense chemistry
Ribonuclease H chemistry

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