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SLC-1 receptor mediates effect of melanin-concentrating hormone on feeding behavior in rat: a structure-activity study.
- Source :
-
The Journal of pharmacology and experimental therapeutics [J Pharmacol Exp Ther] 2001 Oct; Vol. 299 (1), pp. 137-46. - Publication Year :
- 2001
-
Abstract
- Several studies have shown that melanin-concentrating hormone (MCH) is an orexigenic peptide in rat. In the present study, a structure-activity relationship with MCH analogs was performed in rat, both in vitro and in vivo. On rat recombinant SLC-1 receptor, both cAMP inhibition and [(125)I]S36057 binding were measured. In vivo, these analogs were injected intracerebroventricularly in rats and their effects were evaluated upon food intake. First, data obtained with the rat recombinant receptor were highly correlated with those obtained from its human counterpart. Second, agonist potencies in the cAMP assay were also highly correlated with binding affinities. These peptides could be classified into several groups according to their potency at the SLC-1 receptor (from subnanomolar activity to complete inactivity). Indeed, there was a strong correlation between their effects upon food intake and the results obtained at the rat SLC-1 receptor. The present report describes for the first time the rat SLC-1 receptor pharmacology and clearly establishes the relevance of the SLC-1 receptor in feeding behavior.
- Subjects :
- Animals
Cell Line
Cell Membrane drug effects
Cell Membrane metabolism
Cloning, Molecular
Cyclic AMP metabolism
Injections, Intraventricular
Male
Oligopeptides pharmacology
Poly A metabolism
RNA, Messenger biosynthesis
Rats
Rats, Wistar
Recombinant Proteins metabolism
Structure-Activity Relationship
Feeding Behavior drug effects
Hypothalamic Hormones pharmacology
Melanins pharmacology
Pituitary Hormones pharmacology
Receptors, Somatostatin drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3565
- Volume :
- 299
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of pharmacology and experimental therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 11561073