Synthesis and analysis of RNA containing 6-trifluoromethylpurine ribonucleoside.

Authors :: Véliz EA
Stephens OM
Beal PA
Source :: Organic letters [Org Lett] 2001 Sep 20; Vol. 3 (19), pp. 2969-72.
Publication Year :: 2001
Abstract: We report the synthesis of a 5'-DMT-2'-TBDMS-protected phosphoramidite of 6-trifluoromethylpurine ribonucleoside ((TFM)P) and its use in the site-specific incorporation of 6-trifluoromethylpurine into RNA. Properties of (TFM)P-substituted RNA suggest it will be valuable in the study of RNA structure and the binding of RNA-modifying enzymes, particularly the RNA-editing adenosine deaminases. Reaction: see text.

Subjects :: Amides chemistry
Fluorine
Nuclear Magnetic Resonance, Biomolecular
Phosphoric Acids chemistry
Purine Nucleosides chemistry
RNA analysis
RNA chemistry
RNA Probes chemical synthesis
RNA Probes chemistry
RNA Stability
Ribonucleosides chemistry
Purine Nucleosides chemical synthesis
RNA chemical synthesis
Ribonucleosides chemical synthesis

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