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Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp.
- Source :
-
Infection and immunity [Infect Immun] 2001 Oct; Vol. 69 (10), pp. 6264-70. - Publication Year :
- 2001
-
Abstract
- In this study, we evaluated the ability of DNA vaccines encoding the bacterioferritin (BFR) or P39 proteins of Brucella spp. to induce cellular and humoral immune responses and to protect BALB/c mice against a challenge with B. abortus 544. We constructed eukaryotic expression vectors called pCIBFR and pCIP39, encoding BFR or P39 antigens, respectively, and we verified that these proteins were produced after transfection of COS-7 cells. PCIBFR or pCIP39 was injected intramuscularly three times, at 3-week intervals. pCIP39 induced higher antibody responses than did the DNA vector encoding BFR. Both vectors elicited a T-cell-proliferative response and also induced a strong gamma interferon production upon restimulation with either the specific antigens or Brucella extract. In this report, we also demonstrate that animals immunized with these plasmids elicited a strong and long-lived memory immune response which persisted at least 3 months after the third vaccination. Furthermore, pCIBFR and pCIP39 induced a typical T-helper 1-dominated immune response in mice, as determined by cytokine or immunoglobulin G isotype analysis. The pCIP39 delivered by intramuscular injection (but not the pCIBFR or control vectors) induced a moderate protection in BALB/c mice challenged with B. abortus 544 compared to that observed in positive control mice vaccinated with S19.
- Subjects :
- Animals
Antibodies, Bacterial biosynthesis
COS Cells
Carrier Proteins immunology
Cell Division
Chlorocebus aethiops
Cytochrome b Group immunology
Ferritins immunology
Genetic Vectors
Interferon-gamma biosynthesis
Interleukin-5 biosynthesis
Membrane Proteins immunology
Mice
Mice, Inbred BALB C
Spleen cytology
Spleen immunology
T-Lymphocytes cytology
T-Lymphocytes immunology
Bacterial Proteins
Brucella Vaccine immunology
Brucella abortus immunology
Brucellosis prevention & control
Carrier Proteins genetics
Cytochrome b Group genetics
DNA, Bacterial immunology
Ferritins genetics
Membrane Proteins genetics
Periplasmic Binding Proteins
Vaccines, DNA immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 69
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 11553569
- Full Text :
- https://doi.org/10.1128/IAI.69.10.6264-6270.2001