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Production and characterization of monoclonal antibodies to wall-localized peroxidases from corn seedlings.
- Source :
-
Plant physiology [Plant Physiol] 1988; Vol. 88, pp. 1446-53. - Publication Year :
- 1988
-
Abstract
- A library of 22 hybridomas, which make antibodies to soluble wall antigens from the coleoptiles and primary leaves of etiolated corn (Zea mays L.) seedlings, was raised and cloned three times by limit dilution to assure monoclonal growth and stability. Two of these hybridomas made immunoglobulin G antibodies, designated mWP3 and mWP19, which both effectively immunoprecipitated peroxidase activity from crude and partially purified preparations of wall peroxidases. Direct peroxidase-binding assays revealed that both antibodies bound enzymes with peroxidase activity. As judged by immunoblot analyses, mWP3 recognized a Mr 98,000 wall peroxidase with an isoelectric point near 4.2, and mWP19 recognized a Mr 58,000 wall peroxidase. Immunogold localization studies showed both peroxidases are predominately in cell walls.
- Subjects :
- Antibodies, Monoclonal immunology
Blotting, Western
Cell Wall immunology
Cotyledon cytology
Cotyledon enzymology
Cotyledon immunology
Electrophoresis, Polyacrylamide Gel
Hybridomas
Immunoglobulin G biosynthesis
Immunoglobulin G immunology
Immunohistochemistry
Peroxidases metabolism
Peroxidases ultrastructure
Plant Proteins metabolism
Precipitin Tests
Protein Binding
Zea mays immunology
Antibodies, Monoclonal biosynthesis
Cell Wall enzymology
Peroxidases analysis
Plant Proteins analysis
Zea mays enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0889
- Volume :
- 88
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11537437
- Full Text :
- https://doi.org/10.1104/pp.88.4.1446