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Induction of CD4(+) T cell-dependent CD8(+) type 1 responses in humans by a malaria DNA vaccine.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2001 Sep 11; Vol. 98 (19), pp. 10817-22. Date of Electronic Publication: 2001 Aug 28. - Publication Year :
- 2001
-
Abstract
- We assessed immunogenicity of a malaria DNA vaccine administered by needle i.m. or needleless jet injection [i.m. or i.m./intradermally (i.d.)] in 14 volunteers. Antigen-specific IFN-gamma responses were detected by enzyme-linked immunospot (ELISPOT) assays in all subjects to multiple 9- to 23-aa peptides containing class I and/or class II restricted epitopes, and were dependent on both CD8(+) and CD4(+) T cells. Overall, frequency of response was significantly greater after i.m. jet injection. CD8(+)-dependent cytotoxic T lymphocytes (CTL) were detected in 8/14 volunteers. Demonstration in humans of elicitation of the class I restricted IFN-gamma responses we believe necessary for protection against the liver stage of malaria parasites brings us closer to an effective malaria vaccine.
- Subjects :
- Adolescent
Adult
Amino Acid Sequence
Animals
Antigens, Protozoan immunology
Humans
Interferon-gamma biosynthesis
Interleukin-4 analysis
Malaria Vaccines administration & dosage
Malaria, Falciparum immunology
Malaria, Falciparum prevention & control
Molecular Sequence Data
T-Lymphocyte Subsets immunology
Vaccines, DNA administration & dosage
CD4-Positive T-Lymphocytes immunology
DNA, Protozoan immunology
Malaria Vaccines immunology
Plasmodium falciparum immunology
Protozoan Proteins genetics
T-Lymphocytes, Cytotoxic immunology
Vaccines, DNA immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 98
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 11526203
- Full Text :
- https://doi.org/10.1073/pnas.181123498