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Evidence for the Involvement of annexin 6 in the trafficking between the endocytic compartment and lysosomes.
- Source :
-
Experimental cell research [Exp Cell Res] 2001 Sep 10; Vol. 269 (1), pp. 13-22. - Publication Year :
- 2001
-
Abstract
- Annexins are a family of calcium-dependent phospholipid-binding proteins, which have been implicated in a variety of biological processes including membrane trafficking. The annexin 6/lgp120 prelysosomal compartment of NRK cells was loaded with low-density lipoprotein (LDL) and then its transport from this endocytic compartment and its degradation in lysosomes were studied. NRK cells were microinjected with the mutated annexin 6 (anx6(1-175)), to assess the possible involvement of annexin 6 in the transport of LDL from the prelysosomal compartment. The results indicated that microinjection of mutated annexin 6, in NRK cells, showed the accumulation of LDL in larger endocytic structures, denoting retention of LDL in the prelysosomal compartment. To confirm the involvement of annexin 6 in the trafficking and the degradation of LDL we used CHO cells transfected with mutated annexin 6(1-175). Thus, in agreement with NRK cells the results obtained in CHO cells demonstrated a significant inhibition of LDL degradation in CHO cells expressing the mutated form of annexin 6 compared to controls overexpressing wild-type annexin 6. Therefore, we conclude that annexin 6 is involved in the trafficking events leading to LDL degradation.<br /> (Copyright 2001 Academic Press.)
- Subjects :
- Animals
Annexin A6 genetics
Annexin A6 pharmacology
CHO Cells drug effects
CHO Cells metabolism
CHO Cells ultrastructure
Calpain metabolism
Carbocyanines pharmacokinetics
Cell Compartmentation drug effects
Cricetinae
Endocytosis drug effects
Fluorescent Antibody Technique
Fluorescent Dyes pharmacokinetics
Genetic Vectors
Iodine Radioisotopes pharmacology
Leupeptins pharmacology
Lipoproteins, LDL drug effects
Lipoproteins, LDL metabolism
Lysosomes drug effects
Protein Transport drug effects
Spectrin metabolism
Subcellular Fractions metabolism
Transport Vesicles drug effects
Annexin A6 metabolism
Cell Compartmentation physiology
Endocytosis physiology
Lysosomes metabolism
Protein Transport physiology
Transport Vesicles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-4827
- Volume :
- 269
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 11525635
- Full Text :
- https://doi.org/10.1006/excr.2001.5268