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Short report: Antibody responses of mice immunized with a tetravalent dengue recombinant protein subunit vaccine.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2001 Aug; Vol. 65 (2), pp. 159-61. - Publication Year :
- 2001
-
Abstract
- Recombinant proteins containing the B domain of dengue virus serotypes 1-4 fused to the maltose binding protein (MBP) of Escherichia coli were evaluated individually and as a tetravalent vaccine candidate in mice. Sera from mice immunized with monovalent DEN-MBP recombinant protein vaccines developed high titers of serotype homologous antibody in the enzyme-linked immunosorbent assay and the plaque-reduction neutralization test. Cross-reactive antibody titers were either several dilutions lower or not detectable. Sera from mice immunized with the tetravalent DEN subunit vaccine neutralized all 4 DEN viruses in the plaque-reduction neutralization test. The neutralizing antibody titers to each individual serotype were significantly greater than any cross-reactive neutralizing antibody titers induced by the monovalent vaccines, providing evidence that the tetravalent DEN recombinant subunit vaccine produced specific neutralizing antibody to all 4 serotypes of dengue virus.
- Subjects :
- Animals
Bacterial Proteins genetics
Carrier Proteins genetics
Cross Reactions
Dengue Virus genetics
Escherichia coli
Immunization
Maltose-Binding Proteins
Mice
Mice, Inbred BALB C
Neutralization Tests
Recombinant Fusion Proteins immunology
Vaccines, Combined
Vaccines, Subunit immunology
Vaccines, Synthetic
Viral Proteins genetics
Viral Vaccines immunology
ATP-Binding Cassette Transporters
Antibodies, Viral blood
Dengue prevention & control
Dengue Virus immunology
Escherichia coli Proteins
Monosaccharide Transport Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9637
- Volume :
- 65
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 11508393
- Full Text :
- https://doi.org/10.4269/ajtmh.2001.65.159