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Nonlinear focusing of DNA macromolecules.
- Source :
-
Physical review. E, Statistical, nonlinear, and soft matter physics [Phys Rev E Stat Nonlin Soft Matter Phys] 2001 Aug; Vol. 64 (2 Pt 1), pp. 021902. Date of Electronic Publication: 2001 Jul 17. - Publication Year :
- 2001
-
Abstract
- The present paper reports the nonlinear electrophoretic focusing techniques developed after an original idea by Chacron and Slater [Phys. Rev. E 56, 3436 (1997)]. Focusing of DNA molecules is achieved in an alternating nonuniform electric field, created in a wedge gel with hyperbolic boundaries. The fractions separated on such a wedge retained their rectilinear shape during the electrophoresis. Experiments with gel electrophoresis confirm the possibility of a noticeable nonlinear focusing of DNA molecules.
- Subjects :
- Bacteriophage lambda chemistry
Bacteriophage lambda genetics
Computer Simulation
DNA isolation & purification
DNA, Viral chemistry
DNA, Viral isolation & purification
DNA, Viral radiation effects
Electrochemistry methods
Sensitivity and Specificity
DNA chemistry
DNA radiation effects
Electromagnetic Fields
Electrophoresis, Gel, Two-Dimensional methods
Models, Chemical
Models, Molecular
Nonlinear Dynamics
Subjects
Details
- Language :
- English
- ISSN :
- 1539-3755
- Volume :
- 64
- Issue :
- 2 Pt 1
- Database :
- MEDLINE
- Journal :
- Physical review. E, Statistical, nonlinear, and soft matter physics
- Publication Type :
- Academic Journal
- Accession number :
- 11497615
- Full Text :
- https://doi.org/10.1103/PhysRevE.64.021902