Adhesion development and the expression of endothelial nitric oxide synthase.

Authors :: Svinarich DM
Zaher FM
Holmdahl L
Chegini N
Gonik B
Diamond MP
Source :: Infectious diseases in obstetrics and gynecology [Infect Dis Obstet Gynecol] 2001; Vol. 9 (2), pp. 113-6.
Publication Year :: 2001
Abstract: Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions.<br />Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and beta-actin mRNA levels.<br />Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were < or = 3.12 x 10(-4), < or = 3.12 x 10(-4), 6.24 x 10(-4) and 2.5 x 10(-3) attomoles/microl, respectively. Beta-actin mRNA levels for all tissues were between 1.25 x 10(-1) and 6.25 x 10(-2) attomoles/microl.<br />Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.