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Peroxisomal membrane proteins are properly targeted to peroxisomes in the absence of COPI- and COPII-mediated vesicular transport.
- Source :
-
Journal of cell science [J Cell Sci] 2001 Jun; Vol. 114 (Pt 11), pp. 2199-204. - Publication Year :
- 2001
-
Abstract
- The classic model for peroxisome biogenesis states that new peroxisomes arise by the fission of pre-existing ones and that peroxisomal matrix and membrane proteins are recruited directly from the cytosol. Recent studies challenge this model and suggest that some peroxisomal membrane proteins might traffic via the endoplasmic reticulum to peroxisomes. We have studied the trafficking in human fibroblasts of three peroxisomal membrane proteins, Pex2p, Pex3p and Pex16p, all of which have been suggested to transit the endoplasmic reticulum before arriving in peroxisomes. Here, we show that targeting of these peroxisomal membrane proteins is not affected by inhibitors of COPI and COPII that block vesicle transport in the early secretory pathway. Moreover, we have obtained no evidence for the presence of these peroxisomal membrane proteins in compartments other than peroxisomes and demonstrate that COPI and COPII inhibitors do not affect peroxisome morphology or integrity. Together, these data fail to provide any evidence for a role of the endoplasmic reticulum in peroxisome biogenesis.
- Subjects :
- Brefeldin A pharmacology
COP-Coated Vesicles drug effects
Cells, Cultured
Coat Protein Complex I metabolism
Endoplasmic Reticulum drug effects
Endoplasmic Reticulum metabolism
Fibroblasts
Humans
Intracellular Membranes drug effects
Intracellular Membranes metabolism
Lipoproteins biosynthesis
Lipoproteins genetics
Lipoproteins metabolism
Membrane Proteins biosynthesis
Membrane Proteins genetics
Microscopy, Fluorescence
Peroxins
Peroxisomal Biogenesis Factor 2
Peroxisomes drug effects
Protein Transport drug effects
ATP-Binding Cassette Transporters
COP-Coated Vesicles metabolism
Coat Protein Complex I antagonists & inhibitors
Fungal Proteins
Membrane Proteins metabolism
Peroxisomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9533
- Volume :
- 114
- Issue :
- Pt 11
- Database :
- MEDLINE
- Journal :
- Journal of cell science
- Publication Type :
- Academic Journal
- Accession number :
- 11493655
- Full Text :
- https://doi.org/10.1242/jcs.114.11.2199