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Transfer of phospholipids from fat body to lipophorin in Rhodnius prolixus.
- Source :
-
Archives of insect biochemistry and physiology [Arch Insect Biochem Physiol] 1992; Vol. 19 (2), pp. 133-44. - Publication Year :
- 1992
-
Abstract
- 32P-Labeled fat bodies (32P-fat bodies) of Rhodnius prolixus females were incubated in the presence of non radioactive purified lipophorin and the release of radioactivity to the medium was analysed to answer the question of whether lipophorin is a reusable shuttle for phospholipids. The radioactivity found in the medium was associated with lipophorin phospholipids. When the 32P-fat bodies were incubated in the absence of lipophorin, only a small amount of radioactivity was released and it was not associated with lipophorin, indicating that there was no release of pre-labeled 32P-lipophorin by the tissue. Analysis of 32P-phospholipids transferred from fat bodies to the lipophorin particles by thin-layer chromatography revealed a predominance of phosphatidylethanolamine and phosphatidylcholine, with minor amounts of phosphatidylserine, phosphatidylinositol, and sphingomyelin. The transfer of phospholipids to lipophorin was linear with time up to 45 min and the process was inhibited at low temperature and by the metabolic inhibitors azide and fluoride. The transfer of phospholipids from the fat bodies to lipophorin was saturable with respect to the concentration of lipophorin, which was half-maximal at about 8 mg/ml. A directional movement of phospholipids from the fat body to lipophorin was observed. The net gain of phospholipids in 2 h of incubation with fat body was 8.54 nmol per insect, which corresponds to 6.69% of increase in the lipophorin phospholipid content. The rate of 32P-phospholipid transfer from fat body to lipophorin particles varied during the days after a blood meal increasing up to day 10 and then decreasing in parallel with the process of oogenesis.
- Subjects :
- Animals
Autoradiography
Azides pharmacology
Carrier Proteins analysis
Electrophoresis, Polyacrylamide Gel
Fat Body chemistry
Female
Fluorides pharmacology
Hemolymph metabolism
Intestines chemistry
Lipoproteins analysis
Oogenesis
Phosphatidylcholines analysis
Phosphatidylethanolamines analysis
Phospholipids analysis
Phosphorus Radioisotopes
Rabbits
Rhodnius chemistry
Time Factors
Vitellogenins analysis
Biological Transport physiology
Carrier Proteins metabolism
Fat Body metabolism
Insect Proteins metabolism
Intestinal Mucosa metabolism
Lipoproteins metabolism
Phospholipids metabolism
Rhodnius metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0739-4462
- Volume :
- 19
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Archives of insect biochemistry and physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11488301
- Full Text :
- https://doi.org/10.1002/arch.940190206