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Detection of antibodies to Hypoderma lineatum in cattle by Western blotting with recombinant hypodermin C antigen.
- Source :
-
Veterinary parasitology [Vet Parasitol] 2001 Aug 01; Vol. 99 (2), pp. 147-54. - Publication Year :
- 2001
-
Abstract
- The cDNA encoding the entire mature hypodermin C (HC) of Hypoderma lineatum was cloned and expressed in Escherichia coli as a glutathione S-transferase fusion protein using pGEX vector. The recombinant HC protein (rHC) was tested by Western blotting to detect antibodies to H. lineatum in cattle. Western blotting with rHC as antigen clearly differentiated between H. lineatum-infested cattle sera and normal cattle sera. Forty-six out of forty-eight serum samples from cattle in Central Mongolia were positive, whereas all 30 serum samples from cows in Hokkaido, Japan, were negative by Western blotting. The result of Western blotting was identical to that of a previously developed enzyme-linked immunosorbent assay. These data demonstrated that Western blotting, with rHC expressed in E. coli, might be a useful method for the diagnosis of cattle hypodermosis.
- Subjects :
- Animals
Base Sequence
Blotting, Western methods
Blotting, Western veterinary
Cattle
Cattle Diseases immunology
Electrophoresis, Polyacrylamide Gel veterinary
Hypodermyiasis diagnosis
Hypodermyiasis immunology
Recombinant Proteins immunology
Serine Endopeptidases genetics
Antibodies blood
Cattle Diseases diagnosis
Diptera immunology
Hypodermyiasis veterinary
Serine Endopeptidases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-4017
- Volume :
- 99
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Veterinary parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 11470181
- Full Text :
- https://doi.org/10.1016/s0304-4017(01)00457-5