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Antimicrobial peptide buforin I inhibits tissue factor-initiated coagulation.
- Source :
-
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2001 Aug 01; Vol. 392 (1), pp. 3-7. - Publication Year :
- 2001
-
Abstract
- The enhanced extrinsic blood coagulation following septic shock often manifests cardiovascular complications. The upregulated monocytic tissue factor (mTF) was shown to be a primary contributor to the extrinsic hypercoagulation following acute bacterial endotoxin (LPS) infection. A single-stage clotting assay monitors TF-initiated coagulation. We herein demonstrate a novel anticoagulant activity of antimicrobial peptide Buforin I (BF I) in offsetting LPS-induced mTF hypercoagulation in THP-1 cells, which was confirmed in a cell-free in vitro model, showing that BF I effectively blocked rabbit brain thromboplastin (rbTF) procoagulant activity. Upon inclusion of 25 microM BF I into human plasma, the prolonged prothrombin time (PT) was consistent with the depressed TF-initiated coagulation. In a two-stage chromogenic assay monitoring S-2288 hydrolysis, BF I significantly inhibited not only mTF- but also rbTF-catalyzed FVII activation accompanied by the diminished FVIIa formation. The inhibition by BF I of FVII activation accounted for its novel anticoagulant activity in offsetting mTF-initiated hypercoagulation.<br /> (Copyright 2001 Academic Press.)
- Subjects :
- Amino Acid Sequence
Animals
Anti-Infective Agents chemistry
Cell Line
Factor VII metabolism
Humans
In Vitro Techniques
Lipopolysaccharides toxicity
Molecular Sequence Data
Proteins chemistry
Rabbits
Shock, Septic blood
Shock, Septic complications
Thrombosis blood
Thrombosis drug therapy
Thrombosis etiology
Anti-Infective Agents pharmacology
Blood Coagulation drug effects
Proteins pharmacology
Thromboplastin antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 0003-9861
- Volume :
- 392
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Archives of biochemistry and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 11469787
- Full Text :
- https://doi.org/10.1006/abbi.2001.2444