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CD1d-restricted NKT cells: an interstrain comparison.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2001 Aug 01; Vol. 167 (3), pp. 1164-73. - Publication Year :
- 2001
-
Abstract
- CD1d-restricted Valpha14-Jalpha281 invariant alphabetaTCR(+) (NKT) cells are well defined in the C57BL/6 mouse strain, but they remain poorly characterized in non-NK1.1-expressing strains. Surrogate markers for NKT cells such as alphabetaTCR(+)CD4(-)CD8(-) and DX5(+)CD3(+) have been used in many studies, although their effectiveness in defining this lineage remains to be verified. Here, we compare NKT cells among C57BL/6, NK1.1-congenic BALB/c, and NK1.1-congenic nonobese diabetic mice. NKT cells were identified and compared using a range of approaches: NK1.1 expression, surrogate phenotypes used in previous studies, labeling with CD1d/alpha-galactosylceramide tetramers, and cytokine production. Our results demonstrate that NKT cells and their CD4/CD8-defined subsets are present in all three strains, and confirm that nonobese diabetic mice have a numerical and functional deficiency in these cells. We also highlight the hazards of using surrogate phenotypes, none of which accurately identify NKT cells, and one in particular (DX5(+)CD3(+)) actually excludes these cells. Finally, our results support the concept that NK1.1 expression may not be an ideal marker for CD1d-restricted NKT cells, many of which are NK1.1-negative, especially within the CD4(+) subset and particularly in NK1.1-congenic BALB/c mice.
- Subjects :
- Animals
Antigens biosynthesis
Antigens, CD biosynthesis
Antigens, CD1 biosynthesis
Antigens, CD1 metabolism
Antigens, CD1d
Antigens, Surface
Binding Sites immunology
CD24 Antigen
CD3 Complex biosynthesis
CD8 Antigens biosynthesis
Carrier Proteins biosynthesis
Cytokines biosynthesis
Galactosylceramides metabolism
Immunophenotyping
Killer Cells, Natural cytology
Killer Cells, Natural metabolism
L-Selectin biosynthesis
Lectins, C-Type
Lymphocyte Count
Membrane Proteins biosynthesis
Mice
Mice, Congenic
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred NOD
NK Cell Lectin-Like Receptor Subfamily B
Organ Specificity genetics
Organ Specificity immunology
Protein Biosynthesis
Receptors, Antigen, T-Cell, alpha-beta biosynthesis
Receptors, Interleukin-2 biosynthesis
Receptors, NK Cell Lectin-Like
T-Lymphocyte Subsets cytology
T-Lymphocyte Subsets metabolism
Antigens, CD1 genetics
Antigens, Ly
Killer Cells, Natural immunology
Membrane Glycoproteins
Proteins
Species Specificity
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 167
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 11466330
- Full Text :
- https://doi.org/10.4049/jimmunol.167.3.1164