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Increased potential for NAD(P)H-dependent reactive oxygen species production of hepatic subcellular fractions of fish species with in vivo exposure to contaminants.
- Source :
-
Marine environmental research [Mar Environ Res] 2000 Jul-Dec; Vol. 50 (1-5), pp. 57-60. - Publication Year :
- 2000
-
Abstract
- The present study investigated the proposed involvement of contaminant-stimulated reactive oxygen species (ROS) production in disease processes in fish. NAD(P)H-dependent ROS production of subcellular fractions was determined by the iron/EDTA-mediated oxidation of 2-keto-4-methiolbutyric acid. Hepatic cytosolic NADPH-dependent and microsomal NAD(P)H-dependent ROS production were increased 51-160% (P < 0.05) in rainbow trout (Oncorhynchus mykiss) 15 weeks after a single i.p. injection of polychlorobiphenyl (PCB) (100 mg Clophen A50 kg-1 wet wt.). Hepatic microsomal NADH-dependent ROS production was 114% higher in perch (Perca fluviatilis) from PCB-contaminated Lake Järnsjön compared to clean Lake Vänern, Sweden. Hepatic mitochondrial NADH-dependent, cytosolic NADH-dependent and microsomal NADPH-dependent ROS production were variously elevated up to 160% in flounder (Platichthys flesus) at various sites along two pollution transects near to the ports of Rotterdam and Amsterdam, Netherlands. Overall the data indicate increased potential for ROS production in liver of fish exposed to field pollution, and support the hypothesis of oxidative stress as a mechanism of contaminant-mediated disease in fish.
- Subjects :
- Animals
Cytosol drug effects
Cytosol metabolism
Flounder
Liver drug effects
Methionine analogs & derivatives
Methionine metabolism
Microsomes, Liver drug effects
Microsomes, Liver metabolism
Mitochondria, Liver drug effects
Mitochondria, Liver metabolism
NAD metabolism
Netherlands
Oncorhynchus mykiss
Oxidative Stress
Perches
Polychlorinated Biphenyls toxicity
Fishes metabolism
Liver metabolism
NADP metabolism
Reactive Oxygen Species metabolism
Water Pollutants, Chemical toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0141-1136
- Volume :
- 50
- Issue :
- 1-5
- Database :
- MEDLINE
- Journal :
- Marine environmental research
- Publication Type :
- Academic Journal
- Accession number :
- 11460751
- Full Text :
- https://doi.org/10.1016/s0141-1136(00)00043-x