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Effects of baicalein isolated from Scutellaria baicalensis Radix on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide in cultured human umbilical vein endothelial cells.
- Source :
-
Planta medica [Planta Med] 2001 Jun; Vol. 67 (4), pp. 331-4. - Publication Year :
- 2001
-
Abstract
- We examined the effects of various flavonoids isolated from the roots of Scutellaria baicalensis Georgi on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide (SFLLRNPNDKYEPF, TRAP) in cultured human umbilical vein endothelial cells. Thrombin and thrombin receptor agonist peptide induced endothelial leukocyte adhesion molecule-1 (ELAM-1) expression. Intercellular adhesion molecule-1 (ICAM-1) expression was also induced by thrombin, but not by TRAP. Baicalein isolated from Scutellariae Radix inhibited ELAM-1 expression induced by thrombin and thrombin receptor agonist peptide dose-dependently, with 50% inhibitory concentrations (IC50) of 5.53 +/- 1.68 microM and 2.44 +/- 1.08 microM, respectively. Furthermore, baicalein inhibited thrombin-induced ICAM-1 expression with an IC50 of 9.67 +/- 1.28 microM. In addition, baicalein inhibited the expressions of ELAM-1 and ICAM-1 stimulated by protein kinase C (PKC) activator phorbol myristate acetate (PMA).
- Subjects :
- Amino Acid Sequence
Analysis of Variance
Cells, Cultured drug effects
Dose-Response Relationship, Drug
E-Selectin drug effects
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Female
Flavonoids pharmacology
Humans
Intercellular Adhesion Molecule-1 drug effects
Medicine, Chinese Traditional
Plant Roots chemistry
Protein Kinase C antagonists & inhibitors
Protein Kinase C drug effects
Proteins pharmacology
Receptors, Thrombin
Tetradecanoylphorbol Acetate pharmacology
Thrombin pharmacology
Umbilical Veins drug effects
Flavanones
Flavonoids isolation & purification
Plants, Medicinal chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0943
- Volume :
- 67
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Planta medica
- Publication Type :
- Academic Journal
- Accession number :
- 11458449
- Full Text :
- https://doi.org/10.1055/s-2001-14328