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Tween-20 activates and solubilizes the mitochondrial membrane-bound, calmodulin dependent NAD+ finase of Avena sativa L.
- Source :
-
The Journal of membrane biology [J Membr Biol] 2001 Jul 15; Vol. 182 (2), pp. 135-46. - Publication Year :
- 2001
-
Abstract
- Among different treatments assayed, a mix of a nonionic detergent (5% Tween-20) with 0.5 m NaCl was found to solubilize a large part of the calmodulin-dependent NAD+ kinase bound to the inner mitochondrial membrane. It also stimulated its activity by increasing 7 times the maximal velocity. Activity stimulation was also observed with phosphatidylcholine, phosphatidylethanolamine and with reductants (HSO3 and DTT). This solubilized NAD+ kinase and the calmodulin-dependent cytosoluble isoform displayed distinct molecular masses, as well as different kinetic parameters. We propose that solubilization of membrane-bound NAD+ kinase could occur in vivo in Avena sativa and could generate a soluble isoform.
- Subjects :
- Calcium pharmacology
Calcium-Calmodulin-Dependent Protein Kinases metabolism
Calmodulin pharmacology
Calmodulin-Binding Proteins pharmacology
Cell Fractionation
Kinetics
Membrane Proteins
Mitochondria metabolism
Oxidation-Reduction
Phosphotransferases (Alcohol Group Acceptor) drug effects
Seeds
Sodium Chloride pharmacology
Surface-Active Agents pharmacology
Avena enzymology
Phosphotransferases (Alcohol Group Acceptor) metabolism
Polysorbates pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2631
- Volume :
- 182
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of membrane biology
- Publication Type :
- Academic Journal
- Accession number :
- 11447505
- Full Text :
- https://doi.org/10.1007/s0023201-0039-8