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[Isolation and identification of PACE-binding protein rpn4--a new transcription activator, participating in regulation of 26S proteosome and other genes].
- Source :
-
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2001 May-Jun; Vol. 35 (3), pp. 420-31. - Publication Year :
- 2001
-
Abstract
- A new upstream activating sequence (UAS) 5'-GGTGGCAAA-3' was detected in the promoters of 27 out of the 33 proteasomal genes and of several genes related to the ubiquitin-proteasomal system of Saccharomices cerevisiae. The sequence was termed proteasome-associated control element (PACE). Gel retardation assay revealed specific binding of PACE with an extracted protein. The protein (64K) was purified by affinity chromatography and was homogeneous by SDS-PAGE. Microsequencing showed that the protein is Rpn4p. The ability of Rpn4p to activate transcription was demonstrated with constructs containing fragments of the RPN5 and CDC48 gene promoters and reporter cat. Binding to PACE, Rpn4p may act as a common transcription factor on the proteasomal and proteasome-related genes.
- Subjects :
- Cations, Divalent
Chromatography, Affinity
DNA-Binding Proteins metabolism
DNA-Binding Proteins physiology
Genes, Fungal
Promoter Regions, Genetic
Proteasome Endopeptidase Complex
Saccharomyces cerevisiae genetics
Trans-Activators metabolism
Trans-Activators physiology
Transcription Factors metabolism
Transcription Factors physiology
Cysteine Endopeptidases metabolism
DNA-Binding Proteins isolation & purification
Gene Expression Regulation physiology
Multienzyme Complexes metabolism
Saccharomyces cerevisiae Proteins
Trans-Activators isolation & purification
Transcription Factors isolation & purification
Subjects
Details
- Language :
- Russian
- ISSN :
- 0026-8984
- Volume :
- 35
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molekuliarnaia biologiia
- Publication Type :
- Academic Journal
- Accession number :
- 11443924