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Multicentre collaborative study to calibrate IGF-II by bioassay and immunoassay: establishment of the First WHO Reference Reagent.
- Source :
-
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society [Growth Horm IGF Res] 2001 Feb; Vol. 11 (1), pp. 18-23. - Publication Year :
- 2001
-
Abstract
- A preparation of recombinant insulin-like growth factor-II (IGF-II) (NIBSC code 96/538) was compared with local standards in bioassays and immunoassays by eight laboratories in four countries to assess its suitability for use as a World Health Organisation (WHO) reference reagent. Estimates of relative potencies for the bioassays gave a geometric mean of 1.04 (0.94--1.16) microg of local standard per microg of 96/538. Estimates of relative immunological activities by immunoassay gave a geometric mean of 1.15 (0.94--1.38) microg of local standard per microg of 96/538. The study provided evidence that a common standard for rhIGF-II would be helpful and that 96/538 was sufficiently stable to serve as a reference reagent. Accordingly 96/538 was established as the First WHO Reference Reagent for IGF-II, human, recombinant, and assigned a unitage of 5000 units per ampoule and on the basis of the immunoassay results a nominal mass content of 5 microg per ampoule.<br /> (Copyright 2001 Harcourt Publishers Ltd.)
Details
- Language :
- English
- ISSN :
- 1096-6374
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
- Publication Type :
- Academic Journal
- Accession number :
- 11437470
- Full Text :
- https://doi.org/10.1054/ghir.2000.0185