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Correlation between nicotine-induced inhibition of hematopoiesis and decreased CD44 expression on bone marrow stromal cells.
- Source :
-
Blood [Blood] 2001 Jul 15; Vol. 98 (2), pp. 303-12. - Publication Year :
- 2001
-
Abstract
- This study demonstrates that in vivo exposure to cigarette smoke (CS) and in vitro treatment of long-term bone marrow cultures (LTBMCs) with nicotine, a major constituent of CS, result in inhibition of hematopoiesis. Nicotine treatment significantly delayed the onset of hematopoietic foci and reduced their size. Furthermore, the number of long-term culture-initiating cells (LTC-ICs) within an adherent layer of LTBMCs was significantly reduced in cultures treated with nicotine. Although the production of nonadherent mature cells and their progenitors in nicotine-treated LTBMCs was inhibited, this treatment failed to influence the proliferation of committed hematopoietic progenitors when added into methylcellulose cultures. Bone marrow stromal cells are an integral component of the hematopoietic microenvironment and play a critical role in the regulation of hematopoietic stem cell proliferation and self-renewal. Exposure to nicotine decreased CD44 surface expression on primary bone marrow-derived fibroblastlike stromal cells and MS-5 stromal cell line, but not on hematopoietic cells. In addition, mainstream CS altered the trafficking of hematopoietic stem/progenitor cells (HSPC) in vivo. Exposure of mice to CS resulted in the inhibition of HSPC homing into bone marrow. Nicotine and cotinine treatment resulted in reduction of CD44 surface expression on lung microvascular endothelial cell line (LEISVO) and bone marrow-derived (STR-12) endothelial cell line. Nicotine treatment increased E-selectin expression on LEISVO cells, but not on STR-12 cells. These findings demonstrate that nicotine can modulate hematopoiesis by affecting the functions of the hematopoiesis-supportive stromal microenvironment, resulting in the inhibition of bone marrow seeding by LTC-ICs and interfering with stem cell homing by targeting microvascular endothelial cells.
- Subjects :
- Animals
Blood Platelets drug effects
Bone Marrow Cells immunology
Bone Marrow Cells physiology
Cell Adhesion Molecules analysis
Cell Line
Cells, Cultured
Endothelium, Vascular drug effects
Endothelium, Vascular immunology
Flow Cytometry
Hematopoietic Stem Cells cytology
Humans
Mice
Mice, Inbred BALB C
Plants, Toxic
Smoke adverse effects
Stromal Cells drug effects
Stromal Cells physiology
Nicotiana
Umbilical Veins
Bone Marrow Cells drug effects
Hematopoiesis drug effects
Hyaluronan Receptors analysis
Nicotine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 98
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 11435297
- Full Text :
- https://doi.org/10.1182/blood.v98.2.303