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Yeast vectors for integration at the HO locus.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2001 Jun 15; Vol. 29 (12), pp. E59-9. - Publication Year :
- 2001
-
Abstract
- We have constructed new yeast vectors for targeted integration of desired sequences at the Saccharomyces cerevisiae HO locus. Insertion at HO has been shown to have no effect on yeast growth, and thus these integrations should be neutral. One vector contains the KanMX selectable marker, and integrants can be selected by resistance to G418. The other vector contains the hisG-URA3-hisG cassette, and integrants can be selected by uracil prototrophy. Subsequent growth on 5-FOA permits identification of colonies where recombination between the hisG tandem repeats has led to loss of the URA3 marker and return to uracil auxotrophy. We also describe several new bacterial polylinker vectors derived from pUC21 (ampicillin resistance) and pUK21 (kanamycin resistance).
- Subjects :
- Ampicillin Resistance
DNA, Bacterial genetics
DNA, Fungal metabolism
Drug Resistance, Microbial genetics
Escherichia coli genetics
Fungal Proteins genetics
Genes, Fungal genetics
Genetic Markers genetics
Gentamicins pharmacology
Kanamycin Resistance
Molecular Sequence Data
Orotic Acid analogs & derivatives
Orotic Acid pharmacology
Restriction Mapping
Saccharomyces cerevisiae drug effects
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae growth & development
Saccharomyces cerevisiae Proteins
Tandem Repeat Sequences genetics
DNA, Fungal genetics
Deoxyribonucleases, Type II Site-Specific metabolism
Genetic Vectors genetics
Recombination, Genetic genetics
Saccharomyces cerevisiae genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 29
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 11410682
- Full Text :
- https://doi.org/10.1093/nar/29.12.e59