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SPO21 is required for meiosis-specific modification of the spindle pole body in yeast.
- Source :
-
Molecular biology of the cell [Mol Biol Cell] 2001 Jun; Vol. 12 (6), pp. 1611-21. - Publication Year :
- 2001
-
Abstract
- During meiosis II in the yeast Saccharomyces cerevisiae, the cytoplasmic face of the spindle pole body changes from a site of microtubule initiation to a site of de novo membrane formation. These membranes are required to package the haploid meiotic products into spores. This functional change in the spindle pole body involves the expansion and modification of its cytoplasmic face, termed the outer plaque. We report here that SPO21 is required for this modification. The Spo21 protein localizes to the spindle pole in meiotic cells. In the absence of SPO21 the structure of the outer plaque is abnormal, and prospore membranes do not form. Further, decreased dosage of SPO21 leaves only two of the four spindle pole bodies competent to generate membranes. Mutation of CNM67, encoding a known component of the mitotic outer plaque, also results in a meiotic outer plaque defect but does not block membrane formation, suggesting that Spo21p may play a direct role in initiating membrane formation.
- Subjects :
- Alleles
Amino Acid Sequence
Cell Membrane metabolism
Cytoplasm metabolism
Cytoskeletal Proteins genetics
Cytoskeletal Proteins metabolism
Fluorescent Antibody Technique, Indirect
Genotype
Green Fluorescent Proteins
Luminescent Proteins metabolism
Microscopy, Electron
Molecular Sequence Data
Mutation
Phenotype
Plasmids metabolism
Recombinant Fusion Proteins metabolism
Sequence Homology, Amino Acid
Time Factors
Cytoskeletal Proteins physiology
Fungal Proteins metabolism
Meiosis
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 1059-1524
- Volume :
- 12
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular biology of the cell
- Publication Type :
- Academic Journal
- Accession number :
- 11408572
- Full Text :
- https://doi.org/10.1091/mbc.12.6.1611