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Tiagabine treatment and DNA damage in rat astrocytes: an in vitro study by comet assay.
- Source :
-
Neuroscience letters [Neurosci Lett] 2001 Jun 22; Vol. 306 (1-2), pp. 17-20. - Publication Year :
- 2001
-
Abstract
- We studied in vitro the effects of Tiagabine on genomic DNA of cortical rat astrocytes. To evaluate DNA damage, we used a relatively simple technique called Single Cell Gel Electrophoresis or Comet assay. Tiagabine was dissolved in culture medium and added at concentration of 1, 10, 20 and 50 microg/ml on 12-day old cultured astrocytes. In presence of 1 and 10 microg/ml of Tiagabine, no DNA damage was observed after 48 h of treatment. A moderate DNA damage was instead observed for cells exposed to 20 microg/ml of antiepileptic drug. Finally, DNA fragmentation was more evident after treatment with 50 microg/ml of Tiagabine. We conclude that Tiagabine, at the usual recommended doses, does not appear to influence negatively the cortical rat astrocytes, inducing DNA fragmentation only at very high concentrations.
- Subjects :
- Animals
Animals, Newborn
Astrocytes metabolism
Astrocytes pathology
Cells, Cultured metabolism
Cells, Cultured pathology
Cerebral Cortex metabolism
Cerebral Cortex physiopathology
Comet Assay methods
DNA Damage physiology
Dose-Response Relationship, Drug
Genome
Glial Fibrillary Acidic Protein metabolism
Nerve Degeneration chemically induced
Nerve Degeneration metabolism
Nerve Degeneration physiopathology
Rats
Tiagabine
Astrocytes drug effects
Cells, Cultured drug effects
Cerebral Cortex drug effects
DNA Damage drug effects
Epilepsy drug therapy
GABA Agonists toxicity
Nipecotic Acids toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3940
- Volume :
- 306
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 11403947
- Full Text :
- https://doi.org/10.1016/s0304-3940(01)01836-5