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Extraction and quantification of epibatidine in plasma.
- Source :
-
Journal of chromatography. B, Biomedical sciences and applications [J Chromatogr B Biomed Sci Appl] 2001 May 05; Vol. 755 (1-2), pp. 379-82. - Publication Year :
- 2001
-
Abstract
- Epibatidine was extracted from human and mouse plasma into a hexane-isopropanol mixture and back-extracted into a phosphate buffer, pH 2.5, then identified by HPLC isocratically using a CN column and quantified with ultraviolet detection at a fixed wavelength of 214 nm. The percent recovery of epibatidine from spiked plasma samples was 83.6% and the percent extraction was linear between 10 and 1,000 ng/ml. Desipramine was used as the internal standard. For spiked control samples containing 50 and 750 ng/ml, between-day precisions were 20.8 and 7.2% (RSD%), respectively; accuracy was 87.0 and 99.1%, respectively. The limit of detection was 2 ng/ml. Using this method, an intraperitoneal dose of 0.1 mg/kg of epibatidine produced mean levels of 7.3 and 37.1 ng/ml in pooled male and female plasma samples from C57BL/10 J mice, respectively. This is a simple and straightforward procedure by which plasma samples may be analyzed for epibatidine.
- Subjects :
- Animals
Bridged Bicyclo Compounds, Heterocyclic isolation & purification
Bridged Bicyclo Compounds, Heterocyclic pharmacokinetics
Chromatography, High Pressure Liquid methods
Female
Humans
Male
Mice
Mice, Inbred C57BL
Nicotinic Agonists isolation & purification
Nicotinic Agonists pharmacokinetics
Pyridines isolation & purification
Pyridines pharmacokinetics
Reproducibility of Results
Sex Factors
Species Specificity
Bridged Bicyclo Compounds, Heterocyclic blood
Nicotinic Agonists blood
Pyridines blood
Subjects
Details
- Language :
- English
- ISSN :
- 1387-2273
- Volume :
- 755
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Biomedical sciences and applications
- Publication Type :
- Academic Journal
- Accession number :
- 11393730
- Full Text :
- https://doi.org/10.1016/s0378-4347(01)00120-7