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Dopamine receptor regulating factor, DRRF: a zinc finger transcription factor.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2001 Jun 19; Vol. 98 (13), pp. 7558-63. Date of Electronic Publication: 2001 Jun 05. - Publication Year :
- 2001
-
Abstract
- Dopamine receptor genes are under complex transcription control, determining their unique regional distribution in the brain. We describe here a zinc finger type transcription factor, designated dopamine receptor regulating factor (DRRF), which binds to GC and GT boxes in the D1A and D2 dopamine receptor promoters and effectively displaces Sp1 and Sp3 from these sequences. Consequently, DRRF can modulate the activity of these dopamine receptor promoters. Highest DRRF mRNA levels are found in brain with a specific regional distribution including olfactory bulb and tubercle, nucleus accumbens, striatum, hippocampus, amygdala, and frontal cortex. Many of these brain regions also express abundant levels of various dopamine receptors. In vivo, DRRF itself can be regulated by manipulations of dopaminergic transmission. Mice treated with drugs that increase extracellular striatal dopamine levels (cocaine), block dopamine receptors (haloperidol), or destroy dopamine terminals (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) show significant alterations in DRRF mRNA. The latter observations provide a basis for dopamine receptor regulation after these manipulations. We conclude that DRRF is important for modulating dopaminergic transmission in the brain.
- Subjects :
- 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine pharmacology
Amino Acid Sequence
Animals
Autoradiography
Cell Line
Cocaine pharmacology
Conserved Sequence
Corpus Striatum metabolism
Dopamine metabolism
Dopamine Antagonists pharmacology
Down-Regulation
Gene Expression Regulation drug effects
Haloperidol pharmacology
In Situ Hybridization
Kruppel-Like Transcription Factors
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Neuroblastoma
RNA, Messenger analysis
Receptors, Dopamine metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Transcription Factors chemistry
Transfection
Tumor Cells, Cultured
Zinc Fingers
Brain metabolism
Gene Expression Regulation physiology
Neurons metabolism
Receptors, Dopamine genetics
Transcription Factors genetics
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 98
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 11390978
- Full Text :
- https://doi.org/10.1073/pnas.121635798