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Maintenance of TCR clonality in T cells expressing genes for two TCR heterodimers.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2001 Jun 05; Vol. 98 (12), pp. 6824-9. Date of Electronic Publication: 2001 May 29. - Publication Year :
- 2001
-
Abstract
- T cell receptor (TCR) allelic exclusion is believed to be primarily mediated by suppression of further recombination at the TCR locus after the expression of a functional TCR protein. Genetic allelic exclusion has been shown to be leaky for the beta chain and, more commonly, for the alpha chain. Here, we demonstrate an additional mechanism by which T cells can maintain monoclonality. T cells from double TCR transgenic mice express only one or the other of the two available TCRs at the cell surface. This "functional allelic exclusion" is apparently due to control of the TCR assembly process because these T cells express RNA and protein for all four transgenic TCR proteins. Lack of cell surface expression of the second TCR may be controlled by a failure to assemble the TCR heterodimer.
- Subjects :
- Alleles
Dimerization
Flow Cytometry
Humans
RNA, Messenger analysis
Receptors, Antigen, T-Cell, alpha-beta chemistry
Receptors, Antigen, T-Cell, alpha-beta physiology
Reverse Transcriptase Polymerase Chain Reaction
Transgenes
Receptors, Antigen, T-Cell, alpha-beta genetics
T-Lymphocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 98
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 11381132
- Full Text :
- https://doi.org/10.1073/pnas.121179998