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Aspirin effects on gastric epithelial cell proliferation and cytokine expression.
- Source :
-
Microscopy research and technique [Microsc Res Tech] 2001 Jun 01; Vol. 53 (5), pp. 354-9. - Publication Year :
- 2001
-
Abstract
- Aspirin is known to cause gastric injury and to delay ulcer healing. The effects of aspirin on gastric epithelial cell function are heterogeneous; in contrast to injuring the mucosa, aspirin may also act beneficially by inducing adaptation; a mechanism that is poorly understood. We aimed to document the effects of different doses of aspirin on gastric epithelial cell function defined as proliferation, and secretion as well as mRNA expression of cytokines. Furthermore, we studied the effects of aspirin pretreatment on cytokine secretion as a potential element of gastric adaptation. The proliferative activity of three different gastric epithelial cell lines (AGS, KATO III, RGM-1) was assessed by (3)H-thymidine incorporation; secretion of growth factors PDGF-AB and VEGF into culture supernatant was documented by ELISA. mRNA transcripts of both cytokines were quantified by real time RT-PCR. Low doses of aspirin did not alter the proliferative dynamics in two of the three studied cell lines; high doses abolished proliferation. Secretion of PDGF-AB and VEGF increased during the first days of low dose aspirin exposition; higher concentrations led to a depletion of cytokines after an initial liberation in the case of VEGF, mRNA of which was also dose-dependently increased by aspirin. Seven-day pretreatment with low amounts of aspirin did not alter the secretory response of the epithelia caused by higher doses of this drug. The secretion of cytokines and proliferation of gastric epithelial cells are adversely effected by aspirin in a similarly dose-dependent fashion as the intended effects of this drug on platelet function and pain relief.<br /> (Copyright 2001 Wiley-Liss, Inc.)
- Subjects :
- Anti-Inflammatory Agents, Non-Steroidal toxicity
Aspirin toxicity
Cell Division drug effects
Cell Line
Cytokines biosynthesis
Dose-Response Relationship, Drug
Endothelial Growth Factors biosynthesis
Endothelial Growth Factors metabolism
Enzyme-Linked Immunosorbent Assay
Gastric Mucosa cytology
Gastric Mucosa metabolism
Humans
Lymphokines biosynthesis
Lymphokines metabolism
Platelet-Derived Growth Factor biosynthesis
Platelet-Derived Growth Factor metabolism
RNA, Messenger biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Aspirin pharmacology
Cytokines metabolism
Gastric Mucosa drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1059-910X
- Volume :
- 53
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Microscopy research and technique
- Publication Type :
- Academic Journal
- Accession number :
- 11376496
- Full Text :
- https://doi.org/10.1002/jemt.1103