Back to Search
Start Over
Pantothenic acid protects jurkat cells against ultraviolet light-induced apoptosis.
- Source :
-
Free radical biology & medicine [Free Radic Biol Med] 2001 Jun 01; Vol. 30 (11), pp. 1303-10. - Publication Year :
- 2001
-
Abstract
- Human leukemic T lymphocytes (Jurkat cells) were induced to undergo apoptosis by brief irradiation with ultraviolet C light (254 nm). This was accompanied by accumulation of lipid peroxidation products in the form of conjugated dienes, a decrease of total glutathione content, and a shift of its redox state towards the oxidized form. Preincubation of the cells with 1 mM pantothenate resulted in a significant elevation of total glutathione content of the cells, reaching its maximum level, 160% of the control, after 3 h. Similar increase was observed after preincubation with 5 mM N-acetylcysteine, a known precursor of glutathione. Both pantothenic acid and N-acetylcysteine alleviated the ultraviolet-induced decrease of glutathione content, diminished lipid peroxidation, and partly protected the cells against apoptosis produced by ultraviolet irradiation.
- Subjects :
- Acetylcysteine pharmacology
Benzimidazoles
Free Radicals metabolism
Glutathione metabolism
Humans
Jurkat Cells radiation effects
Lipid Peroxidation drug effects
Lipid Peroxidation radiation effects
Oxidation-Reduction
Ultraviolet Rays
Apoptosis drug effects
Apoptosis radiation effects
Cytoprotection drug effects
Jurkat Cells drug effects
Pantothenic Acid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0891-5849
- Volume :
- 30
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Free radical biology & medicine
- Publication Type :
- Academic Journal
- Accession number :
- 11368928
- Full Text :
- https://doi.org/10.1016/s0891-5849(01)00531-7