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Pantothenic acid protects jurkat cells against ultraviolet light-induced apoptosis.

Authors :
Slyshenkov VS
Piwocka K
Sikora E
Wojtczak L
Source :
Free radical biology & medicine [Free Radic Biol Med] 2001 Jun 01; Vol. 30 (11), pp. 1303-10.
Publication Year :
2001

Abstract

Human leukemic T lymphocytes (Jurkat cells) were induced to undergo apoptosis by brief irradiation with ultraviolet C light (254 nm). This was accompanied by accumulation of lipid peroxidation products in the form of conjugated dienes, a decrease of total glutathione content, and a shift of its redox state towards the oxidized form. Preincubation of the cells with 1 mM pantothenate resulted in a significant elevation of total glutathione content of the cells, reaching its maximum level, 160% of the control, after 3 h. Similar increase was observed after preincubation with 5 mM N-acetylcysteine, a known precursor of glutathione. Both pantothenic acid and N-acetylcysteine alleviated the ultraviolet-induced decrease of glutathione content, diminished lipid peroxidation, and partly protected the cells against apoptosis produced by ultraviolet irradiation.

Details

Language :
English
ISSN :
0891-5849
Volume :
30
Issue :
11
Database :
MEDLINE
Journal :
Free radical biology & medicine
Publication Type :
Academic Journal
Accession number :
11368928
Full Text :
https://doi.org/10.1016/s0891-5849(01)00531-7