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Frequency and phenotyping of human immunodeficiency virus (HIV)-specific CD8+ T cells in HIV-infected children, using major histocompatibility complex class I peptide tetramers.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2001 Jun 01; Vol. 183 (11), pp. 1565-73. Date of Electronic Publication: 2001 Apr 27. - Publication Year :
- 2001
-
Abstract
- HLA-A*02 tetramers complexed to human immunodeficiency virus (HIV) Gag SLYNTVATL and HIV Pol ILKEPVHGV peptides were used to characterize HLA class I-restricted CD8(+) T cells in 41 HIV-infected children. The frequencies and the phenotype of specific circulating CD8(+) T cells were determined in whole-blood samples by means of cytometric analysis. Background staining of 13 HLA-A*02-negative patients showed that the frequency of CD8(+) T cells was <0.01%. Of the 28 HLA-A*02-positive patients, blood samples from 26 stained positive at least once the Gag tetramer (mean CD8(+) T cells, 0.87%; range, 0.1%-3.9%), and blood samples from 21 stained positive for the Pol tetramer (mean CD8(+) T cells, 0.59%; range, 0.1%-5.5%). The tetramer-binding cells were CD28(-), CD45RA(-), CD45RO(+), HLA-DR(+), and CD69(-) T lymphocytes. HIV-specific CD8(+) T cells can be detected easily in peripheral blood of HIV-infected children, using HLA tetramers combined with HIV peptides. These cells are memory activated CD28(-)CD8(+) T lymphocytes.
- Subjects :
- Adolescent
Anti-HIV Agents therapeutic use
Antigens, CD analysis
Antigens, Differentiation, T-Lymphocyte analysis
CD28 Antigens analysis
Child
Child, Preschool
Flow Cytometry
Follow-Up Studies
Gene Products, gag analysis
Gene Products, pol analysis
HIV Infections drug therapy
HIV Infections virology
HIV-1 isolation & purification
HLA-A Antigens analysis
HLA-DR Antigens analysis
Humans
Immunophenotyping
Lectins, C-Type
Leukocyte Common Antigens analysis
Lymphocyte Count
Viral Load
CD8-Positive T-Lymphocytes immunology
HIV Infections immunology
HIV-1 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 183
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 11343205
- Full Text :
- https://doi.org/10.1086/320708