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Characterization of the antibody response to pneumococcal glycoconjugates and the effect of heat-labile enterotoxin on IGg subclasses after intranasal immunization.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2001 May 15; Vol. 183 (10), pp. 1494-500. Date of Electronic Publication: 2001 Apr 16. - Publication Year :
- 2001
-
Abstract
- The antibody response to pneumococcal glycoconjugate (Pnc) was characterized by analyzing pneumococcal polysaccharide (PPS)-- and protein carrier-specific IgG subclass profiles and their relationship. Mice were immunized intranasally (inl) or subcutaneously (sc) with Pnc with mutants of Escherichia coli heat-labile enterotoxin, LT-R72 and LT-K63, as mucosal adjuvants. Subcutaneous immunization with Pnc alone induced predominantly IgG1, whereas native PPS administered sc induced very low IgG titers that were exclusively of the IgG3 subclass. Compared with sc immunization with Pnc alone, inl immunization with Pnc and LT mutants induced significantly higher systemic IgG2a, IgG3, and IgA antibodies to both PPS and the carrier, whereas the IgG1 titers were comparable. There also was a significant correlation between PPS- and protein carrier--specific antibody responses for all IgG subclasses. This demonstrates that LT mutants can be used to both enhance and modulate the antibody response to the PS moiety of glycoconjugate vaccines.
- Subjects :
- Adjuvants, Immunologic genetics
Adjuvants, Immunologic pharmacology
Administration, Intranasal
Animals
Bacterial Toxins genetics
Bacterial Toxins pharmacology
Enterotoxins genetics
Enterotoxins pharmacology
Female
Glycoconjugates administration & dosage
Glycoconjugates pharmacology
Mice
Mutation
Pneumococcal Vaccines pharmacology
Polysaccharides, Bacterial administration & dosage
Polysaccharides, Bacterial immunology
Polysaccharides, Bacterial pharmacology
Tetanus Toxoid administration & dosage
Tetanus Toxoid immunology
Tetanus Toxoid pharmacology
Adjuvants, Immunologic administration & dosage
Antibodies, Bacterial biosynthesis
Bacterial Toxins administration & dosage
Enterotoxins administration & dosage
Escherichia coli Proteins
Immunoglobulin G biosynthesis
Pneumococcal Vaccines administration & dosage
Streptococcus pneumoniae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 183
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 11319685
- Full Text :
- https://doi.org/10.1086/320202