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Effect of pre-analytical handling on haematological variables in minipigs.
- Source :
-
Laboratory animals [Lab Anim] 2001 Apr; Vol. 35 (2), pp. 147-52. - Publication Year :
- 2001
-
Abstract
- Pre-analytical handling may be an important determinant of haematological variables, if analysis is delayed. We investigated the effect of anticoagulants, i.e. tripotassium ethylenediamine-tetraacetic acid (EDTA) and citric acid, theophylline, adenosine, dipyridamole (CTAD), storage time (0.5, 1.5, 3.5, 5.5, 7.5, 25.5 and 27.5 h after blood sampling), and storage temperature (5 degrees C and 20 degrees C) on the variation in haemoglobin (HGB), red blood cell count (RBC), haematocrit (HCT), white blood cell count (WBC), and platelet count (PLT) in minipigs. Medians of HGB, RBC, HCT, WBC and PLT were significantly higher in EDTA tubes than in CTAD tubes due to the dilution effect of the anticoagulant. We found a minor significant increase in HCT after 25.5 h in blood stored at 20 degrees C, and at the same time a minor significant increase in WBC in EDTA tubes stored at 20 degrees C. We found a significant decrease in PLT in blood stored at 5 degrees C, especially in EDTA tubes. Minor variations were also observed in HGB and RBC. Our results indicate that PLT should only be measured in tubes placed at room temperature. If HCT or WBC analyses are to be performed on the day after blood sampling, the samples must be stored in a refrigerator until analysis. Our studies underline that time delay before analysis of haematological variables can cause increased variation, and should therefore be limited as far as possible in order to reduce the number of animals needed to make reliable conclusions.
- Subjects :
- Adenosine pharmacology
Animals
Anticoagulants blood
Blood Specimen Collection methods
Blood Specimen Collection standards
Citric Acid pharmacology
Dipyridamole pharmacology
Erythrocyte Count veterinary
Guidelines as Topic
Hematocrit veterinary
Hemoglobins analysis
Leukocyte Count veterinary
Male
Platelet Count veterinary
Random Allocation
Statistics, Nonparametric
Swine
Temperature
Theophylline pharmacology
Time Factors
Anticoagulants pharmacology
Blood Specimen Collection veterinary
Edetic Acid pharmacology
Swine, Miniature blood
Subjects
Details
- Language :
- English
- ISSN :
- 0023-6772
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Laboratory animals
- Publication Type :
- Academic Journal
- Accession number :
- 11315163
- Full Text :
- https://doi.org/10.1258/0023677011911516