Back to Search
Start Over
SDF-1 induces IL-8 production and transendothelial migration of human cord blood-derived mast cells.
- Source :
-
International archives of allergy and immunology [Int Arch Allergy Immunol] 2001 Jan-Mar; Vol. 124 (1-3), pp. 142-5. - Publication Year :
- 2001
-
Abstract
- Background: Mast cell numbers and expression of chemokines are known to increase in the context of angiogenesis and inflammation, but the mechanisms by which this occurs are not understood. Stromal-derived factor-1 (SDF-1) is an important chemokine in angiogenesis and cell migration. The effects of SDF-1 on human mast cells were examined.<br />Methods: Expression of the SDF-1 receptor CXC chemokine receptor 4 (CXCR4) on mast cells was examined by RT-PCR and flow cytometry. The ability of labeled cord blood-derived mast cells to migrate across HUVEC monolayers in response to SDF-1 was determined. The cytokine and chemokine responses of cord blood-derived mast cells to SDF-1 treatment over 24 h were examined by ELISA.<br />Results: Cord blood-derived human mast cells expressed the CXCR4 receptor for SDF-1 and migrated across HUVEC monolayers in response to this chemokine. Treatment of cord blood-derived mast cells with SDF-1 did not induce degranulation or the production of several cytokines but did induce a highly selective IL-8 response.<br />Conclusion: Human mast cells can both migrate across vascular endothelium and produce the pro-angiogenic chemokine IL-8 in response to SDF-1. These responses may be important in angiogenic processes.<br /> (Copyright 2001 S. Karger AG, Basel)
Details
- Language :
- English
- ISSN :
- 1018-2438
- Volume :
- 124
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- International archives of allergy and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 11306951
- Full Text :
- https://doi.org/10.1159/000053693