Back to Search
Start Over
Expression profiling suggested a regulatory role of liver-enriched transcription factors in human hepatocellular carcinoma.
- Source :
-
Cancer research [Cancer Res] 2001 Apr 01; Vol. 61 (7), pp. 3176-81. - Publication Year :
- 2001
-
Abstract
- By using a cDNA array representing 14,000 cDNA clusters, we studied the expression profiles in paired clinical hepatocellular carcinoma (HCC) samples and the distal nontumorous liver tissues from the same patients. Despite the significant heterogeneity among the clinical samples, 72 genes (including 30 novel genes) were down-regulated and 84 genes (including 48 novel genes) were up-regulated in >50% of the cancer samples that were identified. The alterations in gene expression levels were confirmed by Northern blot and reverse-transcription PCR in all of 4 randomly selected genes. It was conspicuous that 21 of 38 hepatocarcinoma (HCC) down-regulated genes studied previously were reportedly regulated by a group of liver-enriched transcription factors (LETFs), and 12 of 36 HCC up-regulated genes studied previously were involved in protein translation. Reexamination of the cDNA array data further revealed that most of the genes known to be regulated by LETFs were down-regulated in at least a portion of the HCC samples. Among the LETFs, the expression level of CCAAT/enhancer-binding protein (C/EBP) alpha was down-regulated in cancer, whereas hepatocyte nuclear factor 1 (HNF-1), HNF-3beta, HNF-4alpha, and HNF-4gamma were up-regulated. The expression profiling thus suggested multiple regulatory pathways involved in HCC, especially that related to LETFs.
- Subjects :
- Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
CCAAT-Enhancer-Binding Protein-alpha physiology
Carcinoma, Hepatocellular metabolism
DNA-Binding Proteins physiology
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Hepatocyte Nuclear Factor 1
Hepatocyte Nuclear Factor 1-alpha
Hepatocyte Nuclear Factor 1-beta
Hepatocyte Nuclear Factor 3-beta
Hepatocyte Nuclear Factor 4
Humans
Liver metabolism
Liver Neoplasms metabolism
Male
Nuclear Proteins physiology
Oligonucleotide Array Sequence Analysis
Phosphoproteins physiology
Carcinoma, Hepatocellular genetics
Liver Neoplasms genetics
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 61
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 11306505