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Helicobacter pylori CagA-positive strains affect oxygen free radicals generation by gastric mucosa.
- Source :
-
Scandinavian journal of gastroenterology [Scand J Gastroenterol] 2001 Mar; Vol. 36 (3), pp. 247-50. - Publication Year :
- 2001
-
Abstract
- Background: Helicobacter pylori plays a key role in production of reactive oxygen metabolites (ROMs). However, the importance of virulent CagA-positive H. pylori strains remains to be determined. The aim of this study was to assess ROMs production in gastric biopsies of patients infected by H. pylori. Results were correlated to CagA status and acute inflammatory infiltration.<br />Methods: Patients undergoing gastroscopy were enrolled. H. pylori infection was assessed by histology and 13C urea breath test. CagA status was assessed through serology. ROMs were assayed in gastric biopsies by luminol-enhanced chemiluminescence (CLS). Gastric mucosal inflammation was histologically graded and neutrophils were individually counted. Macroscopical damage was scored according to a modified Lanza score.<br />Results: 40 out of 60 patients evaluated were H. pylori (HP) positive. Of the 40 infected patients, 24 were CagA-positive. CLS emission was significantly higher in HP-CagA-positive patients than in HP-CagA-negatives and uninfected. ROMs production showed a significant correlation to neutrophil infiltrate in all groups.<br />Conclusions: Gastric mucosa of patients infected by HP-CagA-positive strains is characterized by a higher generation of ROMs and by greater neutrophil counts than that observed in HP-CagA-negative subjects. Since ROMs production is associated with DNA oxidative damage, a long-term stimulation by these strains might be relevant in the pathogenesis of gastric malignancies. Assessment of CagA status might be useful to discriminate patients in which H. pylori eradication is advisable.
- Subjects :
- Bacterial Proteins analysis
Culture Techniques
Female
Free Radicals analysis
Gastritis genetics
Gastritis pathology
Gastritis virology
Gastroscopy
Helicobacter Infections genetics
Helicobacter Infections pathology
Helicobacter pylori genetics
Humans
Male
Probability
Sensitivity and Specificity
Severity of Illness Index
Statistics, Nonparametric
Antigens, Bacterial
Gastric Mucosa metabolism
Gastritis metabolism
Helicobacter Infections metabolism
Helicobacter pylori isolation & purification
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0036-5521
- Volume :
- 36
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Scandinavian journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 11305510
- Full Text :
- https://doi.org/10.1080/003655201750074474