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Ephrin-B reverse signaling is mediated by a novel PDZ-RGS protein and selectively inhibits G protein-coupled chemoattraction.
- Source :
-
Cell [Cell] 2001 Apr 06; Vol. 105 (1), pp. 69-79. - Publication Year :
- 2001
-
Abstract
- Transmembrane B ephrins and their Eph receptors signal bidirectionally. However, neither the cell biological effects nor signal transduction mechanisms of the reverse signal are well understood. We describe a cytoplasmic protein, PDZ-RGS3, which binds B ephrins through a PDZ domain, and has a regulator of heterotrimeric G protein signaling (RGS) domain. PDZ-RGS3 can mediate signaling from the ephrin-B cytoplasmic tail. SDF-1, a chemokine with a G protein-coupled receptor, or BDNF, act as chemoattractants for cerebellar granule cells, with SDF-1 action being selectively inhibited by soluble EphB receptor. This study reveals a pathway that links reverse signaling to cellular guidance, uncovers a novel mode of control for G proteins, and demonstrates a mechanism for selective regulation of responsiveness to neuronal guidance cues.
- Subjects :
- Animals
Biological Assay
Brain-Derived Neurotrophic Factor metabolism
Brain-Derived Neurotrophic Factor pharmacology
Carrier Proteins genetics
Cell Adhesion genetics
Cell Movement drug effects
Cells, Cultured
Chemokine CXCL12
Chemokines, CXC metabolism
Chemokines, CXC pharmacology
Chemotactic Factors pharmacology
Cytoplasm metabolism
Ephrin-B1
Ephrin-B2
Membrane Proteins genetics
Mice
Molecular Sequence Data
Neurons cytology
Neurons drug effects
Neurons metabolism
Protein Structure, Tertiary physiology
RGS Proteins
Sequence Homology, Amino Acid
Signal Transduction drug effects
Transfection
Two-Hybrid System Techniques
Xenopus
Carrier Proteins metabolism
Cell Movement physiology
Chemotactic Factors metabolism
GTP-Binding Proteins
GTPase-Activating Proteins
Heterotrimeric GTP-Binding Proteins metabolism
Membrane Proteins metabolism
Signal Transduction physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0092-8674
- Volume :
- 105
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 11301003
- Full Text :
- https://doi.org/10.1016/s0092-8674(01)00297-5