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Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro.
- Source :
-
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2001 Jan; Vol. 109 (1), pp. 73-8. - Publication Year :
- 2001
-
Abstract
- Human umbilical vein endothelial cells (HUVEC) have previously been shown to synthesize the functional terminal pathway of complement based on the detection by radioimmunoassay of the terminal complement complex (TCC) on coincubated agarose beads. In addition, C7 secretion by these cells in amounts comparable to C3, as well as C7 mRNA, has recently been demonstrated. However, it has not been possible to detect C5-6 and C8 in the fluid phase, and only trace amounts of soluble C9. Against this background we examined whether mRNA for the remaining terminal complement factors was present in HUVEC. By the use of reverse transcription (RT)-polymerase chain reaction (PCR) and Northern blot the presence of mRNA for complement factors C5, C6, C8 and C9 was demonstrated.
- Subjects :
- Base Sequence
Blotting, Northern
Cells, Cultured
Complement C5 genetics
Complement C6 genetics
Complement C8 genetics
Complement C9 genetics
DNA Primers genetics
Endothelium, Vascular metabolism
Humans
Reverse Transcriptase Polymerase Chain Reaction
Umbilical Veins immunology
Umbilical Veins metabolism
Complement System Proteins genetics
Endothelium, Vascular immunology
RNA, Messenger genetics
RNA, Messenger metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0903-4641
- Volume :
- 109
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 11297196
- Full Text :
- https://doi.org/10.1111/j.1600-0463.2001.tb00016.x