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Growth factor-induced angiogenesis in vivo requires specific cleavage of fibrillar type I collagen.
- Source :
-
Blood [Blood] 2001 Apr 15; Vol. 97 (8), pp. 2323-32. - Publication Year :
- 2001
-
Abstract
- The contribution of specific type I collagen remodeling in angiogenesis was studied in vivo using a quantitative chick embryo assay that measures new blood vessel growth into well-defined fibrillar collagen implants. In response to a combination of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), a strong angiogenic response was observed, coincident with invasion into the collagen implants of activated fibroblasts, monocytes, heterophils, and endothelial cells. The angiogenic effect was highly dependent on matrix metalloproteinase (MMP) activity, because new vessel growth was inhibited by both a synthetic MMP inhibitor, BB3103, and a natural MMP inhibitor, TIMP-1. Multiple MMPs were detected in the angiogenic tissue including MMP-2, MMP-13, MMP-16, and a recently cloned MMP-9-like gelatinase. Using this assay system, wild-type collagen was compared to a unique collagenase-resistant collagen (r/r), with regard to the ability of the respective collagen implants to support cell invasion and angiogenesis. It was found that collagenase-resistant collagen constitutes a defective substratum for angiogenesis. In implants made with r/r collagen there was a substantial reduction in the number of endothelial cells and newly formed vessels. The presence of the r/r collagen, however, did not reduce the entry into the implants of other cell types, that is, activated fibroblasts and leukocytes. These results indicate that fibrillar collagen cleavage at collagenase-specific sites is a rate-limiting event in growth factor-stimulated angiogenesis in vivo.
- Subjects :
- Allantois blood supply
Allantois drug effects
Animals
Cells, Cultured drug effects
Chick Embryo
Chorion blood supply
Chorion drug effects
Collagenases metabolism
Endothelium, Vascular cytology
Extracellular Matrix metabolism
Fibroblasts cytology
Hydroxamic Acids pharmacology
Leukocytes cytology
Metalloendopeptidases analysis
Prostheses and Implants
Protease Inhibitors pharmacology
Stromal Cells cytology
Surgical Mesh
Tissue Inhibitor of Metalloproteinase-1 pharmacology
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Collagen metabolism
Endothelial Growth Factors pharmacology
Fibroblast Growth Factor 2 pharmacology
Lymphokines pharmacology
Metalloendopeptidases physiology
Neovascularization, Physiologic drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0006-4971
- Volume :
- 97
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 11290594
- Full Text :
- https://doi.org/10.1182/blood.v97.8.2323