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Nongenomic effect of testosterone on chloride secretion in cultured rat efferent duct epithelia.
- Source :
-
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2001 May; Vol. 280 (5), pp. C1160-7. - Publication Year :
- 2001
-
Abstract
- Short-circuit current (I(sc)) technique was used to investigate the role of testosterone in the regulation of chloride secretion in cultured rat efferent duct epithelia. Among the steroids tested, only testosterone, and to a lesser extent, 5alpha-dihydrotestosterone (5alpha-DHT), reduced the basal and forskolin-induced I(sc) in cultured rat efferent duct epithelia when added to the apical bathing solution. Indomethacin, a 3alpha-hydroxysteroid dehydrogenase, did not affect the inhibitory effect of 5alpha-DHT. The effect of testosterone occurred within 10-20 s upon application and was dose dependent with apparent IC(50) value of 1 microM. The effect was abolished by removal of Cl(-) but not HCO from the normal Krebs-Henseleit solution, suggesting that testosterone mainly inhibited Cl(-) secretion. The efferent duct was found to be most sensitive to testosterone, while the caput and the cauda epididymidis were only mildly sensitive. Cyproterone acetate, a steroidal antiandrogen, or flutamide, a nonsteroidal antiandrogen, did not block the effect of testosterone on the forskolin-induced I(sc), nor did protein synthesis inhibitors, cycloheximide, or actinomycin D. However, pertussis toxin, a G(i) protein inhibitor, attenuated the inhibition of forskolin-induced I(sc) by testosterone. Testosterone caused a dose-dependent inhibition of forskolin-induced rise in cAMP in efferent duct cells. It is suggested that the rapid effect of testosterone was mediated through a membrane receptor that is negatively coupled to adenylate cyclase via G(i) protein. The role of nongenomic action of testosterone in the regulation of electrolyte and fluid transport in the efferent duct is discussed.
- Subjects :
- 1-Methyl-3-isobutylxanthine pharmacology
Acetazolamide pharmacology
Aldosterone pharmacology
Amiloride analogs & derivatives
Amiloride pharmacology
Animals
Calcium Channel Blockers pharmacology
Cell Membrane drug effects
Cell Membrane physiology
Chloride Channels antagonists & inhibitors
Chloride Channels physiology
Colforsin pharmacology
Cyclic AMP metabolism
Dexamethasone pharmacology
Dihydrotestosterone pharmacology
Epithelial Cells drug effects
Ion Channels antagonists & inhibitors
Kinetics
Male
Phlorhizin pharmacology
Protein Synthesis Inhibitors pharmacology
Rats
Rats, Sprague-Dawley
Testis cytology
ortho-Aminobenzoates pharmacology
Chlorides metabolism
Epithelial Cells physiology
Ion Channels physiology
Testis physiology
Testosterone pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0363-6143
- Volume :
- 280
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 11287329
- Full Text :
- https://doi.org/10.1152/ajpcell.2001.280.5.C1160