Isolation and characterization of a protein from Mendole (Spicara maena) eggs that binds to DNA and inhibits its replication as well as its acid precipitation.

Authors :: Kouretas D
Mamuris Z
Stamatis C
Rodriguez-Burgos A
Tsamis V
Neofitou C
Source :: In vivo (Athens, Greece) [In Vivo] 2001 Jan-Feb; Vol. 15 (1), pp. 49-52.
Publication Year :: 2001
Abstract: Proteins that bind and protect nucleic acids from acid precipitation have been characterized from human and mouse plasma. In the present study, one protein from Mendole (Spicara maena) eggs was purified to homogeneity, by means of acetone fractionation and Sephadex G-100 gel filtration. The protein inhibited DNA replication, exerted by various DNA polymerases. Amino-acid sequence analysis in the amino terminus revealed a unique sequence. Its possible physiological role is discussed.

Subjects :: Acids
Amino Acid Sequence
Animals
Cell Fractionation
Chemical Precipitation
DNA-Binding Proteins chemistry
Molecular Sequence Data
Nucleic Acids isolation & purification
Oncorhynchus mykiss
Perciformes
DNA Replication drug effects
DNA-Binding Proteins isolation & purification
DNA-Binding Proteins pharmacology
Ovum chemistry

Tools