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Blood purine and energy status in rats with colitis.
- Source :
-
Digestive diseases and sciences [Dig Dis Sci] 2001 Feb; Vol. 46 (2), pp. 443-8. - Publication Year :
- 2001
-
Abstract
- Colitis reduces the blood and tissue levels of adenosine deaminase and adenylate deaminase. Whether this has any effect on blood purines remains to be determined. The aim of this study was to measure the adenylate pool, substrates of the above enzymes, and energy status in blood from rats with colitis. Colitis was induced by intrarectal administration of acetic acid and followed over a period of seven days. The levels of ATP, ADP, AMP, adenosine, inosine, and uric acid were analyzed by HPLC, and energy status was estimated. Myeloperoxidase was used as a marker of colitis. Concentrations of ATP, ADP, AMP and adenosine decreased during days 1-5, whereas energy status decreased on day 2. The concentrations of inosine, uric acid, and hemoglobin remained unaltered, whereas colonic myeloperoxidase activity increased. These, findings demonstrate colitis-induced reduction of the circulating purines, which may be due to their enhanced usage for the repair of the inflamed colon.
- Subjects :
- Acetic Acid
Adenosine analysis
Adenosine blood
Adenosine Diphosphate analysis
Adenosine Diphosphate blood
Adenosine Monophosphate analysis
Adenosine Monophosphate blood
Adenosine Triphosphate analysis
Adenosine Triphosphate blood
Animals
Biomarkers analysis
Biomarkers blood
Chromatography, High Pressure Liquid
Colitis chemically induced
Colitis pathology
Hemoglobins analysis
Inosine analysis
Inosine blood
Male
Peroxidase analysis
Peroxidase blood
Purines analysis
Rats
Rats, Wistar
Time Factors
Uric Acid analysis
Uric Acid blood
Colitis metabolism
Disease Models, Animal
Energy Metabolism
Purines blood
Subjects
Details
- Language :
- English
- ISSN :
- 0163-2116
- Volume :
- 46
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Digestive diseases and sciences
- Publication Type :
- Academic Journal
- Accession number :
- 11281197
- Full Text :
- https://doi.org/10.1023/a:1005629605443