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Mechanism of the photocatalytic inactivation of Lactobacillus casei phage PL-1 by titania thin film.
- Source :
-
Current microbiology [Curr Microbiol] 2001 Mar; Vol. 42 (3), pp. 184-9. - Publication Year :
- 2001
-
Abstract
- The mechanism of the inactivation of Lactobacillus casei phage PL-1 suspended in a phosphate buffer by black-light (BL) -catalytic titanium dioxide (TiO2) thin film was studied. Generation of both superoxide anions (O2-) and hydroxyl radicals (*OH) was confirmed in the aqueous medium in which TiO2 film was settled with BL irradiation under gentle shaking. With BL-irradiation alone without TiO2 film, only O2- was generated to some extent. The genome DNA inside the phage particles was found to be fragmented by the treatment of PL-1 phages with BL-catalytic TiO2 film. The phage inactivation by BL-catalytic TiO2 film was inhibited by the addition of albumin in a concentration-dependent manner. BL-catalytic TiO2 film was considered to cause primarily the damage to the capsid protein through the generation of active oxygen species such as *OH, followed by damage to the genome DNA inside the phage particles.
- Subjects :
- Antiviral Agents chemistry
Capsid radiation effects
Catalysis
DNA Damage
DNA, Viral metabolism
DNA, Viral radiation effects
Electrophoresis, Agar Gel
Hydroxyl Radical metabolism
Oxidants, Photochemical
Oxidation-Reduction
Photochemistry
Reactive Oxygen Species metabolism
Serum Albumin, Bovine pharmacology
Siphoviridae genetics
Siphoviridae radiation effects
Siphoviridae ultrastructure
Superoxides metabolism
Titanium chemistry
Ultraviolet Rays
Antiviral Agents pharmacology
Lacticaseibacillus casei virology
Siphoviridae drug effects
Titanium pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0343-8651
- Volume :
- 42
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 11270652
- Full Text :
- https://doi.org/10.1007/s002840010201