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Induction of E-selectin after partial hepatectomy promotes metastases to liver in mice.
- Source :
-
The Journal of surgical research [J Surg Res] 2001 Apr; Vol. 96 (2), pp. 197-203. - Publication Year :
- 2001
-
Abstract
- Background: The liver is the most frequent site of tumor metastasis. It has been suggested that partial hepatectomy promotes liver metastasis of malignant disease and that expression of E-selectin, a cell adhesion molecule, plays roles in tumor metastasis. However, no reports are available concerning the expression of E-selectin after hepatectomy.<br />Methods: In the present study, we used BALB/c mice subjected to 30% partial hepatectomy after injection of 1 x 10(4) colon 26 cells to determine the effects of partial hepatectomy on tumor metastasis to liver. E-Selectin expression within the liver after partial hepatectomy was evaluated using reverse transcription polymerase chain reaction and Western blotting. In addition, we injected polyclonal antibody to E-selectin into mice in which partial hepatectomy had augmented liver metastasis.<br />Results: Mice subjected to partial hepatectomy had significantly increased numbers of liver metastases (sham operation, 1.5 +/- 2.0, vs partial hepatectomy, 35.5 +/- 19.3; P < 0.001). Expression of E-selectin mRNA within the liver was markedly increased 4 h after partial hepatectomy, but subsequently decreased at 24 h. E-Selectin protein was detected 8 h after hepatectomy, but subsequently decreased at 24 h as measured by Western blotting. Mice subjected to intraperitoneal injection of neutralizing antibody after operation had significantly decreased numbers of liver metastases (phosphate-buffered saline, 20.6 +/- 9.2, P < 0.05, and normal IgG, 18.0 +/- 8.0, P < 0.05, compared with polyclonal antibody to E-selectin, 5.6 +/- 4.8).<br />Conclusion: Induction of E-selectin by partial hepatectomy promotes hematogenous liver metastasis. Our findings can be applied to surgical treatment of liver tumor to reduce the recurrence of liver metastasis after hepatectomy by inhibiting E-selectin-mediated adhesion using reagents to E-selectin.<br /> (Copyright 2001 Academic Press.)
- Subjects :
- Animals
Antibodies pharmacology
E-Selectin genetics
E-Selectin immunology
Injections, Intravenous
Liver Neoplasms pathology
Male
Mice
Mice, Inbred BALB C
Neoplasm Transplantation
Portal Vein
Postoperative Period
RNA, Messenger metabolism
Tumor Cells, Cultured
E-Selectin metabolism
Hepatectomy methods
Liver Neoplasms etiology
Liver Neoplasms secondary
Subjects
Details
- Language :
- English
- ISSN :
- 0022-4804
- Volume :
- 96
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of surgical research
- Publication Type :
- Academic Journal
- Accession number :
- 11266273
- Full Text :
- https://doi.org/10.1006/jsre.2001.6095